Lending Library of Kits

Biotechnology
  • DNA Extraction
  • Forensic DNA Fingerprinting Kit by Bio-Rad
  • Crime Scene Investigator PCR Basics Kit by BioRad
  • GMO investigator Kit by BioRad
  • pGLO Bacterial Transformation Kit by BioRad
  • Green Flourescent Protein Chromotography Kit by BioRad
  • Comparitive Proteomics Kit I: Protein Profiler Module by BioRad
  • Comparitive Proteomics Kit II: Western Blot Module by BioRad
  • Protein Synthesis Kinestetic Module
  • Other equipment available
Nanoscience
  • LED Colorstrip Kit from MrSEC
  • Color Changing Beads/UV Light Module
  • Memory Metal Module
  • Stain Resistant Fabric Module
Microelectronics
  • Embedded Systems - Programming Microcontrollers
  • LED Colorstrip Kit from MrSEC
Alternative Energy

Wind Trainer from US Didactic
Solar Trainer from US Didactic
Hydrogen Fuel Cell Trainer from US Didactic
Microbial Fuel Cell Kit by Carolina
Shake Flashlight Wave simulation Kit

Microscopy

Tutorial on SEM operation

DNA Extraction

Extract your own DNA with this easy to use kit.

Forensic DNA Fingerprinting Kit by Bio-Rad

DNA evidence assists in criminal, missing persons, mass disaster, and paternity cases. It can be used to identify a perpetrator or exonerate the innocent. Using real DNA as evidence, your students play the role of crime scene investigator to figure out for themselves "Who done it?"

The six DNA samples in this kit are plasmids engineered to mimic the natural variations in DNA that exist between one human being and another. One DNA sample has been collected from a "crime scene" and five samples have been obtained from various "suspects." Each sample is digested using a mixture of two DNA restriction enzymes, which generates a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by agarose gel electrophoresis and visualized using Bio-Rad's revolutionary Fast Blast DNA stain.

Crime Scene Investigator PCR Basics Kit by BioRad

DNA profiling determines the exact genotype of a DNA sample, and distinguishes one human being from another by identifying a DNA "bar code" that is unique to every individual. This powerful tool assists in investigations of crime scenes, missing persons, mass disasters, immigration disputes, and paternity testing. Crime scenes often contain tiny biological specimens (such as blood, semen, hairs, saliva, bones, pieces of skin) from which DNA can be extracted. The reason DNA profiling is so useful for forensic scientists is that a definitive DNA profile can be obtained from a minute biological sample, using the power of DNA amplification afforded by PCR.

The Crime Scene Investigator PCR Basics kit is an introductory polymerase chain reaction (PCR) kit that allows students to simulate DNA profiling as commonly used in forensic laboratories. The laboratory activity is designed to introduce the concepts of PCR, which is widely used in forensics, diagnostics, and archeological procedures. The laboratory activity is performed in two lesson periods without the need for complex genomic DNA extraction steps.

GMO investigator Kit by BioRad

Genetically modified (GM) foods do not require labeling as such in the U.S., and foods with <5% content from genetically modified organisms (GMOs) can be labeled "GMO free". In much of Europe and Asia, GM foods require labeling even if they contain >1% GM content. The GMO Investigator kit is designed to allow students to test their favorite foods for the presence of GM content.

Students engage in a complete investigation in which they gather sample food items from the grocery store, extract DNA from the samples, amplify the DNA using polymerase chain reaction (PCR), and use agarose gel electrophoresis to identify the presence or absence of amplified GMO sequences.

The GMO Investigator kit uses PCR and DNA electrophoresis to test for the presence of two different GMO-associated DNA sequences: the 35S promoter of the cauliflower mosaic virus and the terminator of the nopaline synthase gene of Agrobacterium tumefaciens. These DNA sequences are present in >85% of the GM crops that are approved for distribution worldwide. As a control for the integrity of the plant DNA extracted from food, PCR is used to amplify a section of the photosystem II chloroplast gene that is common to most higher plants.

The kit allows a guided inquiry approach to this exercise. Students conduct sophisticated scientific procedures, employing multiple levels of controls that allow them to assess the validity of their results. They determine the presence or absence of GMO sequences in their food samples and answer the questions: did we successfully extract DNA; did our PCR work as expected, and do we have GM content?

Regardless of where your students stand on the GM debate, won't they be interested to know how much of the corn- or soy-based foods they eat have been genetically modified?

pGLO Bacterial Transformation Kit by BioRad

Genetic engineering is the process of manipulating the genetic material of an organism - often to include the DNA from a foreign organism. In this activity, students transform bacteria by introducing a gene from the bioluminescent jellyfish Aequorea victoria. They use the same procedure for creating "designer proteins" that has led to the explosion of new health treatments, agricultural applications, and environmental solutions.

Bio-Rad's exclusive pGLO plasmid is constructed with the jellyfish gene that encodes Green Fluorescent Protein (GFP), an antibiotic-resistance gene that encodes &#946;-lactamase protein and the AraC gene encoding a regulator protein that turns the GFP gene on and off. Bacteria transformed with pGLO are selected by ampicillin resistance, and, when induced to express GFP, the bugs glow fluorescent green under UV light!

With this activity, students analyze the growth of bacteria on various media and examine the roles of external and internal factors in gene regulation. Gene expression in all organisms is carefully regulated to allow adaptation to differing conditions and to prevent wasteful production of proteins. Bacterial genes encoding the enzymes needed to metabolize the simple sugar arabinose are a perfect example. A promoter region upstream of these genes acts as a molecular on/off switch that regulates their expression. The genes are activated only when arabinose is present in the environment. Bio-Rad's pGLO plasmid incorporates the arabinose promoter, but the genes involved in the breakdown of arabinose have been replaced with the jellyfish gene encoding GFP. When bacteria transformed with pGLO plasmid are grown in the presence of arabinose, the GFP gene switches on, causing the bacteria to express GFP and fluoresce brilliant green. When students genetically reengineer bacteria with the genes from a bioluminescent jellyfish, they never forget the central mantra of molecular biology.

Green Flourescent Protein Chromotography Kit by BioRad

Use the Green Fluorescent Protein Purification (GFP) kit to take your students through the process of creating a new product - from discovery in the laboratory to biomanufacturing to market. In biotechnology research and industry, transformation is the first step in producing genetically engineered "designer" proteins. Genetically engineered proteins have unlimited applications - from medicines to treat human diseases to powerful enzymes incorporated into nonpolluting laundry detergents - but they must be overexpressed, mass produced, and purified to be marketable.

Students begin this activity with the pGLO Bacterial Transformation kit, which is purchased separately. A colony of transformed bacteria are placed in liquid cultures overnight then lysed to release their cellular contents. GFP is purified from the bacterial contaminants using the hydrophobic interaction chromatography (HIC) columns provided in this kit. Green Fluorescent Protein (GFP) is extremely hydrophobic compared to bacterial proteins. This unique characteristic of GFP enables the purification of GFP from bacterial cell proteins using HIC columns. When placed in a buffer containing a high concentration of salt, the HIC matrix selectively binds hydrophobic GFP molecules while allowing the bacterial proteins to pass right through the column. Then, simply lowering the salt concentration of the buffer causes GFP to elute from the column in pure form.

The unique fluorescent property of GFP allows real time monitoring of the extraction and purification processes. pGLO transformation and GFP purification together are key processes used in biotechnology to produce and purify designer proteins with commercial or research value.

Use this kit to show your students the relevance of the science they learn in the classroom to science in their lives. Encourage them to imagine the possibilities and to think critically and creatively.

Comparitive Proteomics Kit I: Protein Profiler Module by BioRad

The Protein Profiler Module guides students through the thought processes involved in a laboratory-based scientific investigation. This module allows students to employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function. Students learn to use SDS-PAGE to generate protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles to test the hypothesis that protein profiles can be indicators of evolutionary relatedness.

This kit allows your students to explore evolution at the molecular level within the context of the central molecular framework of biology:

DNA RNA Protein Trait = Phenotype

Changes in proteins can reflect changes in the gene pool. Actin and myosin are the major muscle proteins essential for locomotion and survival in all animals. Muscle consists mainly of actin and myosin, but numerous other proteins also comprise muscle tissue. The structures and functions of actin and myosin have remained relatively stable or conserved in all animals over evolutionary time. However, other muscle proteins exhibit considerable variation even among closely related species. Detectable variations between organisms' protein profiles reflect physiological adaptations to different environments, but they originate as random DNA mutations. Such mutation events, if favorable, persist through the natural selection process and contribute to the evolution of species - with new specialized functions.

Mutation Variation Specialization Speciation = Evolution

This is an open-ended inquiry-based kit. Students make predictions about their results in prelab activities using internet databases and published phylogenetic information. They generate novel results and apply their findings directly to the problem of solving evolutionary relationships by constructing cladograms (phylogenetic trees). From their gel data, they build up a tree and assign each organism a branch. Students can decide whether their results support their predictions.

Comparitive Proteomics Kit II: Western Blot Module by BioRad

Myosin is a major muscle protein essential for locomotion and survival in all animals. The essential structure and function of myosin has remained relatively stable or "conserved" in all animals over evolutionary time. However, differences in the molecular weights of the myosin light chain proteins of different species are detectable via western blotting, enabling students to hypothesize about how these variations relate to their evolutionary relationships.

In the first part of this laboratory (Comparative Proteomics Kit I: Protein Profiler Module), students use SDS-PAGE to generate protein profiles and visualize the unique arrays of proteins comprising the muscle tissues from different fish samples. From their polyacrylamide protein gel results, students make educated guesses as to the identities of the proteins. However, based on relative molecular masses alone, these inferences remain guesses.

In this, the second part of the laboratory, students use western blotting to specifically identify myosin light chain from the hundreds of other proteins that comprise the muscle cell extracts of closely and distantly related species of fish. The protocol involves transferring the protein bands in polyacrylamide gels to a membrane and then using an anti-myosin light chain antibody to precisely identify which protein in each species' profile is myosin light chain.

Western blotting is used extensively in research to determine the presence of specific proteins, to quantify their expression levels, and to determine whether they have undergone genetic or posttranslational modifications. This surefire method categorically identifies proteins of interest based on two distinguishing features: molecular mass and antibody binding specificity.

Using Internet-based bioinformatics databases, students can compare their experimentally determined results to actual protein sequence data derived from DNA and RNA sequences and consider whether variations in myosins between species are due to "genetic" or "epigenetic" factors.

Protein Synthesis Kinestetic Module

This Kinestetic activities gets students out of their seat 'becoming' the DNA strand. The students put on a t-shirt that represents part of the process of protein synthesis and move about simulating the process.

Other Equipment Available

Gel electrophoresis chambers, powerpacks, Thermocycler, mini centrifuge.

LED Colorstrip Kit from MrSEC

The vivid colors of light emitting diodes (LEDs) are an engaging blend of art, science, and technology. These eye-catching devices are revolutionizing lighting and display technologies because, relative to incandescent lights, LEDs are more compact, robust, and directional, more energy efficient and less heat generating, and are more rapidly switched on and off. Because the semiconducting solids used in LEDs can be grown virtually an atomic layer at a time, these materials represent an exciting application of nanotechnology that draws upon chemistry, physics and engineering principles. The ICE LED Color Strip Kit has been developed to acquaint teachers and students with some of the scientific and engineering concepts underpinning these remarkable lighting devices.

Color Changing Beads/UV Light Module

Discover the world of UV light and why wearing sunscreen in important! This kit examines different brands of sunscreens using UV color changing beads. The students discover what SPF means how effective their sunscreen is in protecting them against harmful UV rays.

Memory Metal Module

Discover the world of memory metal. This metal discovered by the Navy is a Nickle and Titanium alloy that has 'magical' properties. The metal has been trained to set the atoms in a particular place but can be manipulated at different temperatures.

Stain Resistant Fabric Module

Nanotex is cutting edge fabric used in clothing, cars, and other products. This stain-resistant fabric repels many substances with its hydrophobic interactions. This technology is was discovered simply by looking at mother nature, more specifically the gecko's foot, who uses Nano hairs to cling from a glass ceiling!

Embedded Systems - Programming Microcontrollers

Program blinking lights with this easy to use (and free) downloadable program! Embedded systems are used in todays products. Examples are the garage door sensor, the computer chip in your car, the microelectronic device that runs your cell phone and even your ipod. Students learn the basics of programming these devices with a microcontroller built with LED lights.

LED Colorstrip Kit from MrSEC

The vivid colors of light emitting diodes (LEDs) are an engaging blend of art, science, and technology. These eye-catching devices are revolutionizing lighting and display technologies because, relative to incandescent lights, LEDs are more compact, robust, and directional, more energy efficient and less heat generating, and are more rapidly switched on and off. Because the semiconducting solids used in LEDs can be grown virtually an atomic layer at a time, these materials represent an exciting application of nanotechnology that draws upon chemistry, physics and engineering principles. The ICE LED Color Strip Kit has been developed to acquaint teachers and students with some of the scientific and engineering concepts underpinning these remarkable lighting devices.

Wind Trainer from US Didactic

Demonstrate on a tabletop scale the conversion of energy from wind to electricity. The wind power plant generator (turbine) can be setup with different blade configurations, and the source of airflow is adjustable. All required circuit modules and measuring instruments are included. Some variables to test include: straight or curved blades, angle, wind speed, and number of blades. Students use these variable to light a lightbulb, turn a motor, or measure voltage.

Solar Trainer from US Didactic

A collection of photovoltaic cells, circuit modules, and instrumentation with an experimental platform suitable for classroom demonstrations and student experiments. Some variable to test include: parallel vs series circuitry, number of solar panels, sun intensity, and time of day. Students use these variable to light a lighbulb, turn a motor, or measure voltage.

Hydrogen Fuel Cell Trainer from US Didactic

Turn water into an alternative energy source! Perform classroom demonstrations and oversee student experiments using an electrolyser and fuel cell. With this kit, students will be able to light a lightbulb, run a motor, and measure voltage using water as their source of enegy. All required electronics and metering are included together with a rugged base plate.

Microbial Fuel Cell Kit by Carolina

Advanced - For experienced high school and college classes; requires some technical skill.
• Investigate cellular respiration
• Explore an alternative fuel source
• See microbes in a new light

Use this high-quality fuel cell in an exciting cross-curricular lab that explores cellular respiration, electrical currents, microbiology, and the need for alternative fuels. The fuel cell uses the metabolic activity of ordinary baker's yeast to create a small electric current; its design is based on original research done by Dr. Peter Bennetto, formerly of King's College, London. Chemicals arrive dry, in premeasured amounts.

Shake Flashlight Wave Simulation Kit

Measure the conversion of mechanical motion to energy by shaking a flashlight! By shaking the flashlight, a magnet on the inside moves through an electrical coil and generates electricity. Students can measure the energy and the dissipation of that energy with this kit.

Oops! It appears your browser is no longer supported.

You have a few options from this point. The first and most recommended option would be to download one of the browsers below. If that is not possible, the second suggestion would be to update your current browser, you can follow the update link below for that. Otherwise, the site will still function in its current state, but in a limited capacity.

New Browsers

Updates

×